| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Geobacillus thermodenitrificans | AGGAAAGGCAGCGGAAGT | AGCTGCACCGACTTGGAT | 58.84 | 58.93 | 154 | 141 |
100.00%
|
50.00%
|
| Primer 2 | Geobacillus thermodenitrificans | GCGAACGCAATCAACGCA | AAGCGTTTCGTTGCCGAC | 60.13 | 59.37 | 220 | 138 |
100.00%
|
50.00%
|
| Primer 3 | Geobacillus thermodenitrificans | ACTTGACGCTGCTTGGCA | AAAGTCCGGTTGGCGAGT | 60.20 | 59.17 | 161 | 138 |
100.00%
|
50.00%
|
| Primer 4 | Geobacillus thermodenitrificans | AAGATTCCGAGCTGCGCA | ACGGCAACACCTTCGTCTC | 59.73 | 60.30 | 243 | 138 |
100.00%
|
50.00%
|
| Primer 5 | Geobacillus thermodenitrificans | GCGAACGCAATCAACGCA | AGCGTTTCGTTGCCGACA | 60.13 | 60.59 | 219 | 137 |
100.00%
|
50.00%
|
| Primer 6 | Geobacillus thermodenitrificans | TGGCAACGGCTCCAATGT | AGCGTCGCCACTTTCACA | 59.88 | 59.89 | 279 | 137 |
100.00%
|
50.00%
|
| Primer 7 | Geobacillus thermodenitrificans | TTGCGCACCGGTGTCAT | ACGGTTCCAACGCCAACA | 59.93 | 60.12 | 187 | 137 |
100.00%
|
50.00%
|
| Primer 8 | Geobacillus thermodenitrificans | AGAAACAGGGATCGACGCC | ACGGTTCCAACGCCAACA | 59.78 | 60.12 | 227 | 137 |
100.00%
|
50.00%
|
| Primer 9 | Geobacillus thermodenitrificans | CGCACCGGTGTCATCAATG | ACGGTTCCAACGCCAACA | 59.57 | 60.12 | 184 | 137 |
100.00%
|
50.00%
|
| Primer 10 | Geobacillus thermodenitrificans | ACGGGATCGAGGTGCAAA | TCGCGAAACCCGACAACA | 58.93 | 59.89 | 259 | 137 |
100.00%
|
50.00%
|